Hotspot

psaA-ycf3

LSC · 48255-49131 · 877 bp

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Pi 0.00942
MarkerSeek score 67.20
Primer available yes
Label psaA-ycf3

Feature Metadata

label_name
psaA-ycf3
feature_type
igs
region
LSC
length_bp
877
variable_sites
8
pi
0.00942
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
67.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psaA-ycf3_p1 GCCCATTCCTCGAAAGATGT ATCAAGCTGCCGAGTATTGG 50.0 50.0 58.0 58.0 1027-1055 bp 1.0 82.1
psaA-ycf3_p2 TGAGAAATGACCCGGTTTGG ATCAAGCTGCCGAGTATTGG 50.0 50.0 58.1 58.0 1046-1074 bp 1.0 81.6
psaA-ycf3_p3 GCCCATTCCTCGAAAGATGT CGATCAAGCTGCCGAGTATT 50.0 50.0 58.0 58.1 1029-1057 bp 1.0 81.3
psaA-ycf3_p4 GCCCATTCCTCGAAAGATGT TTGAAGATCACGAGGCGTTT 50.0 45.0 58.0 57.8 956-984 bp 1.0 81.3
psaA-ycf3_p5 GCCCATTCCTCGAAAGATGT TTGGTTGAAGATCACGAGGC 50.0 50.0 58.0 58.2 960-988 bp 1.0 81.1