Hotspot

petN-psbM

LSC · 34521-35714 · 1194 bp

Back to Fockea

Pi 0.00754
MarkerSeek score 63.10
Primer available yes
Label petN-psbM

Feature Metadata

label_name
petN-psbM
feature_type
igs
region
LSC
length_bp
1194
variable_sites
9
pi
0.00754
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
63.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petN-psbM_p1 TCGTAGTGTGGGGAAGAAGT TCATTCTAGTTCCTACCGCCT 50.0 47.6 58.0 58.0 1288 bp 1.0 72.3
petN-psbM_p2 TTTCCCTTTCGCTCGTAGTG TCATTCTAGTTCCTACCGCCT 50.0 47.6 57.9 58.0 1300 bp 1.0 71.7
petN-psbM_p3 TCGTAGTGTGGGGAAGAAGT TCTAGTTCCTACCGCCTTTCT 50.0 47.6 58.0 57.9 1284 bp 1.0 71.7
petN-psbM_p4 CGTAGTGTGGGGAAGAAGTG TCATTCTAGTTCCTACCGCCT 55.0 47.6 57.9 58.0 1287 bp 1.0 71.7
petN-psbM_p5 ACATTTTCCCTTTCGCTCGT TCATTCTAGTTCCTACCGCCT 45.0 47.6 58.1 58.0 1304 bp 1.0 71.5