Hotspot

rps16-trnQ-UUG

LSC · 6107-7337 · 1231 bp

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Pi 0.0163
MarkerSeek score 58.70
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
1231
variable_sites
20
pi
0.0163
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
58.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 1304-1327 bp 1.0 79.6
rps16-trnQ-UUG_p2 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 1315-1338 bp 1.0 79.0
rps16-trnQ-UUG_p3 AAGTCGCACGTTGCTTTCTA TTCGGAGGTTCGAATCCTTC 45.0 50.0 58.1 57.3 1308-1331 bp 1.0 74.8
rps16-trnQ-UUG_p4 AAGTCGCACGTTGCTTTCTA TAAGGCAACGGGTTTTGGTC 45.0 50.0 58.1 58.7 1334-1357 bp 1.0 74.7
rps16-trnQ-UUG_p5 ATCATGTCCTTCAAGTCGCA GAGGTTCGAATCCTTCCGTC 45.0 55.0 57.2 58.1 1316-1339 bp 1.0 74.5