Hotspot

trnH-GUG-psbA

LSC · 76-350 · 275 bp

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Pi 0.0836
MarkerSeek score 78.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
275
variable_sites
23
pi
0.0836
unique_haplotype_count
2
primer_available
yes
alignment_reliability
1.00
markerseek_score
78.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG TTCCCTCTAGACCTAGCTGC 55.0 55.0 58.1 57.9 344 bp 1.0 83.7
trnH-GUG-psbA_p2 GATCCACTTGGCTACATCCG GCGCTAACCTTGGTATGGAA 55.0 50.0 58.1 58.0 391 bp 1.0 83.4
trnH-GUG-psbA_p3 CAATCCACTGCCTTGATCCA GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 405 bp 1.0 83.2
trnH-GUG-psbA_p4 AATCCACTGCCTTGATCCAC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 404 bp 1.0 83.2
trnH-GUG-psbA_p5 ACAATCCACTGCCTTGATCC GCGCTAACCTTGGTATGGAA 50.0 50.0 57.9 58.0 406 bp 1.0 83.2