Hotspot

rpl14-rpl16

LSC · 83462-83592 · 131 bp

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Pi 0.0709
MarkerSeek score 74.60
Primer available yes
Label rpl14-rpl16

Feature Metadata

label_name
rpl14-rpl16
feature_type
igs
region
LSC
length_bp
131
variable_sites
9
pi
0.0709
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.97
markerseek_score
74.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl14-rpl16_p1 TCTGCTACATTTAAATAGGTTTGAGA TCACTAGCAGCGTCCAAAAT 30.8 45.0 56.7 57.5 216-220 bp 1.0 44.8
rpl14-rpl16_p2 CTGCTACATTTAAATAGGTTTGAGA TTCACTAGCAGCGTCCAAAA 32.0 45.0 55.4 57.8 216-220 bp 1.0 44.8
rpl14-rpl16_p3 TCTGCTACATTTAAATAGGTTTGAGA TTCACTAGCAGCGTCCAAAA 30.8 45.0 56.7 57.8 217-221 bp 1.0 44.7
rpl14-rpl16_p4 TCTGCTACATTTAAATAGGTTTGAGA TTTCACTAGCAGCGTCCAAA 30.8 45.0 56.7 57.8 218-222 bp 1.0 44.7
rpl14-rpl16_p5 CTGCTACATTTAAATAGGTTTGAGA TTTCACTAGCAGCGTCCAAA 32.0 45.0 55.4 57.8 217-221 bp 1.0 44.7