Hotspot

rpl36-rps8

LSC · 82001-82493 · 493 bp

Back to Flueggea

Pi 0.0292

MarkerSeek score 68.60

Primer available yes

Label rpl36-rps8

Feature Metadata

label_name: rpl36-rps8
feature_type: igs
region: LSC
length_bp: 493
variable_sites: 14
pi: 0.0292
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rpl36-rps8_p1	ATTCGCCCTCGTCTACGTAT	AATGACAGATCGAGAGGCTC	50.0	50.0	58.4	56.8	615-625 bp	1.0	71.0
rpl36-rps8_p2	AATTCGCCCTCGTCTACGTA	AATGACAGATCGAGAGGCTC	50.0	50.0	58.6	56.8	616-626 bp	1.0	69.3
rpl36-rps8_p3	TAATTCGCCCTCGTCTACGT	AATGACAGATCGAGAGGCTC	50.0	50.0	58.6	56.8	617-627 bp	1.0	69.3
rpl36-rps8_p4	GCCCTCGTCTACGTATCAGT	AATGACAGATCGAGAGGCTC	55.0	50.0	58.7	56.8	611-621 bp	1.0	68.7
rpl36-rps8_p5	ATAATTCGCCCTCGTCTACG	AATGACAGATCGAGAGGCTC	50.0	50.0	57.3	56.8	618-628 bp	1.0	68.6