Hotspot

petA-psbJ

LSC · 65147-66276 · 1130 bp

Back to Flueggea

Pi 0.0324
MarkerSeek score 76.90
Primer available yes
Label petA-psbJ

Feature Metadata

label_name
petA-psbJ
feature_type
igs
region
LSC
length_bp
1130
variable_sites
29
pi
0.0324
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.79
markerseek_score
76.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
petA-psbJ_p1 CATTACGTGTCCAAGGCCTT ACTGGTATTCCTGTGATCGG 50.0 50.0 58.2 56.7 1150-1321 bp 1.0 74.2
petA-psbJ_p2 CATTACGTGTCCAAGGCCTT CTGGTATTCCTGTGATCGGT 50.0 50.0 58.2 56.7 1149-1320 bp 1.0 74.2
petA-psbJ_p3 GACCCATTACGTGTCCAAGG ACTGGTATTCCTGTGATCGG 55.0 50.0 58.3 56.7 1154-1325 bp 1.0 73.5
petA-psbJ_p4 GACCCATTACGTGTCCAAGG CTGGTATTCCTGTGATCGGT 55.0 50.0 58.3 56.7 1153-1324 bp 1.0 73.5
petA-psbJ_p5 TTACGTGTCCAAGGCCTTTT CTGGTATTCCTGTGATCGGT 45.0 50.0 57.6 56.7 1147-1318 bp 1.0 72.8