Hotspot

ycf4-cemA

LSC · 62366-63247 · 882 bp

Back to Flueggea

Pi 0.0248

MarkerSeek score 57.60

Primer available yes

Label ycf4-cemA

Feature Metadata

label_name: ycf4-cemA
feature_type: igs
region: LSC
length_bp: 882
variable_sites: 9
pi: 0.0248
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.41
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf4-cemA_p1	TTGGCCTATTTCTTGCGTGT	AGATCCACCAGGGCAAAAAG	45.0	50.0	58.1	58.1	491-1000 bp	1.0	83.6
ycf4-cemA_p2	TTGGCCTATTTCTTGCGTGT	GAGATCCACCAGGGCAAAAA	45.0	50.0	58.1	58.1	492-1001 bp	1.0	83.6
ycf4-cemA_p3	TTGGCCTATTTCTTGCGTGT	GATCCACCAGGGCAAAAAGA	45.0	50.0	58.1	58.1	490-999 bp	1.0	83.6
ycf4-cemA_p4	TTGGCCTATTTCTTGCGTGT	AAAAAGAGATCCACCAGGGC	45.0	50.0	58.1	57.8	497-1006 bp	1.0	82.4
ycf4-cemA_p5	TTGGCCTATTTCTTGCGTGT	CCACCAGGGCAAAAAGACTA	45.0	50.0	58.1	57.7	487-996 bp	1.0	81.9