Hotspot

rps14-psaB

LSC · 39255-39371 · 117 bp

Back to Flueggea

Pi 0.147

MarkerSeek score 77.10

Primer available yes

Label rps14-psaB

Feature Metadata

label_name: rps14-psaB
feature_type: igs
region: LSC
length_bp: 117
variable_sites: 16
pi: 0.147
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.93
markerseek_score: 77.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps14-psaB_p1	AATGGTAAAGGGGCTCCTCT	AATTTCCTGGCGCGGATATT	50.0	45.0	58.0	58.0	706-714 bp	1.0	83.4
rps14-psaB_p2	TCCTACCCTCTCCCTCTTCT	AATTTCCTGGCGCGGATATT	55.0	45.0	58.0	58.0	995 bp	0.5	59.8
rps14-psaB_p3	TCCTACCCTCTCCCTCTTCT	CCCGGCTGGTTAAATGCTAT	55.0	50.0	58.0	58.0	1504 bp	0.5	59.8
rps14-psaB_p4	TCTCCTACCCTCTCCCTCTT	AATTTCCTGGCGCGGATATT	55.0	45.0	58.0	58.0	997 bp	0.5	59.8
rps14-psaB_p5	TCTCCTACCCTCTCCCTCTT	CCCGGCTGGTTAAATGCTAT	55.0	50.0	58.0	58.0	1506 bp	0.5	59.8