Hotspot

psbZ-trnG-GCC

LSC · 37701-38445 · 745 bp

Back to Flueggea

Pi 0.0557

MarkerSeek score 77.60

Primer available yes

Label psbZ-trnG-GCC

Feature Metadata

label_name: psbZ-trnG-GCC
feature_type: igs
region: LSC
length_bp: 745
variable_sites: 30
pi: 0.0557
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 0.72
markerseek_score: 77.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psbZ-trnG-GCC_p1	CTCCTGATGGTTGGTCAAGT	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	57.1	57.8	704-896 bp	1.0	78.7
psbZ-trnG-GCC_p2	CTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	707-899 bp	1.0	76.4
psbZ-trnG-GCC_p3	TCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	50.0	50.0	56.8	57.8	705-897 bp	1.0	76.4
psbZ-trnG-GCC_p4	TCTTCTCCTGATGGTTGGTCA	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	58.4	57.8	708-900 bp	1.0	74.6
psbZ-trnG-GCC_p5	TTCTCCTGATGGTTGGTCAAG	CGTCTTCTCCTTGGCAAAGA	47.6	50.0	57.6	57.8	706-898 bp	1.0	74.2