Hotspot

trnS-GCU-trnG-UCC

LSC · 9317-10091 · 775 bp

Back to Flueggea

Pi 0.172
MarkerSeek score 90.50
Primer available yes
Label trnS-GCU-trnG-UCC

Feature Metadata

label_name
trnS-GCU-trnG-UCC
feature_type
igs
region
LSC
length_bp
775
variable_sites
117
pi
0.172
unique_haplotype_count
2
primer_available
yes
alignment_reliability
0.88
markerseek_score
90.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnS-GCU-trnG-UCC_p1 CAATCCGACGCTTTAGTCCA TGAACCGAGAGATCCCTTAAC 50.0 47.6 58.0 56.8 800-881 bp 1.0 74.6
trnS-GCU-trnG-UCC_p2 AATCCGACGCTTTAGTCCAC TGAACCGAGAGATCCCTTAAC 50.0 47.6 58.0 56.8 799-880 bp 1.0 74.6
trnS-GCU-trnG-UCC_p3 CAATCCGACGCTTTAGTCCA TGAACCGAGAGATCCCTTAACT 50.0 45.5 58.0 58.3 800-881 bp 1.0 73.6
trnS-GCU-trnG-UCC_p4 AATCCGACGCTTTAGTCCAC TGAACCGAGAGATCCCTTAACT 50.0 45.5 58.0 58.3 799-880 bp 1.0 73.6
trnS-GCU-trnG-UCC_p5 AGAACCGGAAAGAGAGGGAT TGAACCGAGAGATCCCTTAAC 50.0 47.6 57.7 56.8 858-939 bp 1.0 72.6