Hotspot

ccsA

SSC · 112720-113697 · 978 bp

Back to Flemingia

Pi 0.00477

MarkerSeek score 37.90

Primer available yes

Label ccsA

Feature Metadata

label_name: ccsA
feature_type: gene
region: SSC
length_bp: 978
variable_sites: 9
pi: 0.00477
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 37.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA_p1	TAGACACGCTGCTCTTAGGA	CTCGACGAAATGGGTGGAAT	50.0	50.0	57.9	58.0	1777-1787 bp	1.0	80.3
ccsA_p2	TAGACACGCTGCTCTTAGGA	CGAAATGGGTGGAATGGCTA	50.0	50.0	57.9	57.9	1772-1782 bp	1.0	80.0
ccsA_p3	AAATTGGTAGACACGCTGCT	CTCGACGAAATGGGTGGAAT	45.0	50.0	57.8	58.0	1784-1794 bp	1.0	79.8
ccsA_p4	GGTAGACACGCTGCTCTTAG	CTCGACGAAATGGGTGGAAT	55.0	50.0	57.8	58.0	1779-1789 bp	1.0	79.7
ccsA_p5	GTAGACACGCTGCTCTTAGG	CTCGACGAAATGGGTGGAAT	55.0	50.0	57.8	58.0	1778-1788 bp	1.0	79.7