Hotspot

ccsA-ndhD

SSC · 113698-113943 · 246 bp

Back to Flemingia

Pi 0.0247
MarkerSeek score 66.30
Primer available yes
Label ccsA-ndhD

Feature Metadata

label_name
ccsA-ndhD
feature_type
igs
region
SSC
length_bp
246
variable_sites
10
pi
0.0247
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.96
markerseek_score
66.30

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ccsA-ndhD_p1 AGGGCTACATAGTTATGGTTCA CGTCTGACAAGGTTGAAGCT 40.9 50.0 56.3 58.1 314-324 bp 1.0 54.6
ccsA-ndhD_p2 AGGGCTACATAGTTATGGTTCA TCGTCTGACAAGGTTGAAGC 40.9 50.0 56.3 58.1 315-325 bp 1.0 54.6
ccsA-ndhD_p3 AGGGCTACATAGTTATGGTTCAT CGTCTGACAAGGTTGAAGCT 39.1 50.0 56.8 58.1 314-324 bp 1.0 50.6
ccsA-ndhD_p4 AGGGCTACATAGTTATGGTTCAT TCGTCTGACAAGGTTGAAGC 39.1 50.0 56.8 58.1 315-325 bp 1.0 50.6
ccsA-ndhD_p5 GGGCTACATAGTTATGGTTCA CGTCTGACAAGGTTGAAGCT 42.9 50.0 54.8 58.1 313-323 bp 1.0 50.4