Hotspot

rps3-rps19

LSC · 82843-83364 · 522 bp

Back to Flemingia

Pi 0.0143

MarkerSeek score 48.50

Primer available yes

Label rps3-rps19

Feature Metadata

label_name: rps3-rps19
feature_type: igs
region: LSC
length_bp: 522
variable_sites: 13
pi: 0.0143
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.98
markerseek_score: 48.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rps19_p1	CAATTCCCTCGACACCTGAG	GGGCCCCAATTGGTAGAAAA	55.0	50.0	58.0	58.1	1118-1155 bp	1.0	83.2
rps3-rps19_p2	CAATTCCCTCGACACCTGAG	GTCGGACAAGTGGGAAATGT	55.0	50.0	58.0	58.1	1257-1294 bp	1.0	82.4
rps3-rps19_p3	CAATTCCCTCGACACCTGAG	TCGGACAAGTGGGAAATGTG	55.0	50.0	58.0	58.1	1256-1293 bp	1.0	82.4
rps3-rps19_p4	CAATTCCCTCGACACCTGAG	ATTACCTTCTGGGGAGGTCC	55.0	55.0	58.0	58.1	1306-1343 bp	1.0	82.3
rps3-rps19_p5	CAATTCCCTCGACACCTGAG	TGGGGAGGTCCGTTTGATAT	55.0	50.0	58.0	58.1	1297-1334 bp	1.0	82.3