Hotspot

rpl33-rps18

LSC · 66758-67005 · 248 bp

Back to Flemingia

Pi 0.0565
MarkerSeek score 80.10
Primer available yes
Label rpl33-rps18

Feature Metadata

label_name
rpl33-rps18
feature_type
igs
region
LSC
length_bp
248
variable_sites
23
pi
0.0565
unique_haplotype_count
3
primer_available
yes
alignment_reliability
1.00
markerseek_score
80.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl33-rps18_p1 CGACACAATACGCCTAGTCG TCAATTCTGTCCCCCGATTG 55.0 50.0 58.5 57.6 421-447 bp 1.0 77.8
rpl33-rps18_p2 CGACACAATACGCCTAGTCG ATTCTGTCCCCCGATTGGAT 55.0 50.0 58.5 58.5 418-444 bp 1.0 77.4
rpl33-rps18_p3 CGACACAATACGCCTAGTCG CAATTCTGTCCCCCGATTGG 55.0 55.0 58.5 58.6 420-446 bp 1.0 76.3
rpl33-rps18_p4 CGACACAATACGCCTAGTCG AATTCTGTCCCCCGATTGGA 55.0 50.0 58.5 58.7 419-445 bp 1.0 75.5
rpl33-rps18_p5 GACACAATACGCCTAGTCGA TCAATTCTGTCCCCCGATTG 50.0 50.0 57.2 57.6 420-446 bp 1.0 75.4