Hotspot

trnC-GCA-rpoB

LSC · 33938-34770 · 833 bp

Back to Flemingia

Pi 0.0072

MarkerSeek score 46.60

Primer available yes

Label trnC-GCA-rpoB

Feature Metadata

label_name: trnC-GCA-rpoB
feature_type: igs
region: LSC
length_bp: 833
variable_sites: 10
pi: 0.0072
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 46.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnC-GCA-rpoB_p1	CCGGATTTGAACTGGGGAAA	CCTTCATTTCCACCCCCAAG	50.0	55.0	58.1	58.4	918-930 bp	1.0	77.4
trnC-GCA-rpoB_p2	CCGGATTTGAACTGGGGAAA	ATTCCTTCATTTCCACCCCC	50.0	50.0	58.1	57.4	921-933 bp	1.0	76.5
trnC-GCA-rpoB_p3	AACCTCTTGTCGATAAGGCG	CCTTCATTTCCACCCCCAAG	50.0	55.0	57.7	58.4	942-954 bp	1.0	75.6
trnC-GCA-rpoB_p4	AAAAGGATTTGCAGTCCCCC	CCTTCATTTCCACCCCCAAG	50.0	55.0	58.4	58.4	900-912 bp	1.0	75.2
trnC-GCA-rpoB_p5	AACCTCTTGTCGATAAGGCG	ATTCCTTCATTTCCACCCCC	50.0	50.0	57.7	57.4	945-957 bp	1.0	74.8