Hotspot

petN-trnC-GCA

LSC · 32983-33866 · 884 bp

Back to Flemingia

Pi 0.00377

MarkerSeek score 62.00

Primer available yes

Label petN-trnC-GCA

Feature Metadata

label_name: petN-trnC-GCA
feature_type: igs
region: LSC
length_bp: 884
variable_sites: 6
pi: 0.00377
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 62.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-trnC-GCA_p1	TTAAAGCAGCCCAAGCAAGA	TTTCCCCAGTTCAAATCCGG	45.0	50.0	58.0	58.1	947-1010 bp	1.0	80.3
petN-trnC-GCA_p2	GCCCAAGCAAGACTTACGAT	TTTCCCCAGTTCAAATCCGG	50.0	50.0	58.3	58.1	939-1002 bp	1.0	78.3
petN-trnC-GCA_p3	TTAAAGCAGCCCAAGCAAGA	GGGGGACTGCAAATCCTTTT	45.0	50.0	58.0	58.4	965-1028 bp	1.0	78.1
petN-trnC-GCA_p4	TCTTCCCCATACTACGAGGG	TTTCCCCAGTTCAAATCCGG	55.0	50.0	57.6	58.1	991-1054 bp	1.0	77.3
petN-trnC-GCA_p5	TTAAAGCAGCCCAAGCAAGA	TATCTATTTGGCGGCATGGC	45.0	50.0	58.0	58.5	998-1061 bp	1.0	77.2