Hotspot

trnT-GGU-trnE-UUC

LSC · 30101-30860 · 760 bp

Back to Flemingia

Pi 0.00574

MarkerSeek score 63.50

Primer available yes

Label trnT-GGU-trnE-UUC

Feature Metadata

label_name: trnT-GGU-trnE-UUC
feature_type: igs
region: LSC
length_bp: 760
variable_sites: 8
pi: 0.00574
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnT-GGU-trnE-UUC_p1	GAACCGATGACTTACGCCTT	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.0	58.3	858-872 bp	1.0	79.2
trnT-GGU-trnE-UUC_p2	GAACCGATGACTTACGCCTT	TGTCCTGAACCACTAGACGA	50.0	50.0	58.0	57.7	837-851 bp	1.0	79.1
trnT-GGU-trnE-UUC_p3	CCATGGCGTTACTCTACCAC	GCTGCCTCCTTGAAAGAGAG	55.0	55.0	58.1	58.3	837-851 bp	1.0	78.8
trnT-GGU-trnE-UUC_p4	CCATGGCGTTACTCTACCAC	TGTCCTGAACCACTAGACGA	55.0	50.0	58.1	57.7	816-830 bp	1.0	78.7
trnT-GGU-trnE-UUC_p5	TGGCGTTACTCTACCACTGA	GCTGCCTCCTTGAAAGAGAG	50.0	55.0	58.1	58.3	834-848 bp	1.0	78.6