Hotspot

trnG-GCC-psbZ

LSC · 24934-25686 · 753 bp

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Pi 0.0103
MarkerSeek score 51.60
Primer available yes
Label trnG-GCC-psbZ

Feature Metadata

label_name
trnG-GCC-psbZ
feature_type
igs
region
LSC
length_bp
753
variable_sites
11
pi
0.0103
unique_haplotype_count
3
primer_available
yes
alignment_reliability
0.97
markerseek_score
51.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnG-GCC-psbZ_p1 CGTCTTCTCCTTGGCAAAGA CGAAGGTTGGTCAAGTAACA 50.0 45.0 57.8 55.6 876-903 bp 1.0 62.9
trnG-GCC-psbZ_p2 ATAACTAAGTGGGGGCGGAT CGAAGGTTGGTCAAGTAACA 50.0 45.0 58.2 55.6 913-940 bp 1.0 62.7
trnG-GCC-psbZ_p3 CGTCTTCTCCTTGGCAAAGA CGAAGGTTGGTCAAGTAACAA 50.0 42.9 57.8 56.4 876-903 bp 1.0 61.1
trnG-GCC-psbZ_p4 ATAACTAAGTGGGGGCGGAT CGAAGGTTGGTCAAGTAACAA 50.0 42.9 58.2 56.4 913-940 bp 1.0 60.9
trnG-GCC-psbZ_p5 TAACTAAGTGGGGGCGGATA CGAAGGTTGGTCAAGTAACA 50.0 45.0 57.5 55.6 912-939 bp 1.0 60.3