Hotspot

trnH-GUG-psbA

LSC · 93-418 · 326 bp

Back to Flemingia

Pi 0.0191

MarkerSeek score 37.20

Primer available yes

Label trnH-GUG-psbA

Feature Metadata

label_name: trnH-GUG-psbA
feature_type: igs
region: LSC
length_bp: 326
variable_sites: 8
pi: 0.0191
unique_haplotype_count: 3
primer_available: yes
alignment_reliability: 0.71
markerseek_score: 37.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnH-GUG-psbA_p1	GATCCACTTGGCTACATCCG	CTGCGATTGAAGCTCCATCT	55.0	50.0	58.1	58.0	283-379 bp	1.0	84.9
trnH-GUG-psbA_p2	CAATCCACTGCCTTGATCCA	CTGCGATTGAAGCTCCATCT	50.0	50.0	57.9	58.0	297-393 bp	1.0	84.8
trnH-GUG-psbA_p3	AATCCACTGCCTTGATCCAC	CTGCGATTGAAGCTCCATCT	50.0	50.0	57.9	58.0	296-392 bp	1.0	84.8
trnH-GUG-psbA_p4	ACAATCCACTGCCTTGATCC	CTGCGATTGAAGCTCCATCT	50.0	50.0	57.9	58.0	298-394 bp	1.0	84.8
trnH-GUG-psbA_p5	GATCCACTTGGCTACATCCG	GGTGTAGCTGGTGTATTCGG	55.0	55.0	58.1	58.1	713-809 bp	1.0	83.4