Hotspot

trnR-ACG-trnN-GUU

IRb · 109334-109998 · 665 bp

Back to Flacourtia

Pi 0.00924

MarkerSeek score 69.00

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 665
variable_sites: 10
pi: 0.00924
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 69.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	AACAGATCCAGTGGAGACGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.1	58.0	814-842 bp	1.0	83.1
trnR-ACG-trnN-GUU_p2	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	778-806 bp	1.0	82.1
trnR-ACG-trnN-GUU_p3	AACAGATCCAGTGGAGACGA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	58.1	58.2	765-793 bp	1.0	82.1
trnR-ACG-trnN-GUU_p4	AACAGATCCAGTGGAGACGA	GCTGTTAACTGACTGGTCGT	50.0	50.0	58.1	57.8	778-806 bp	1.0	82.0
trnR-ACG-trnN-GUU_p5	AACAGATCCAGTGGAGACGA	CTGGTCGTAGGTTCGAATCC	50.0	55.0	58.1	57.8	766-794 bp	1.0	81.7