Hotspot

psaI-ycf4

LSC · 59824-60271 · 448 bp

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Pi 0.0149

MarkerSeek score 68.90

Primer available yes

Label psaI-ycf4

Feature Metadata

label_name: psaI-ycf4
feature_type: igs
region: LSC
length_bp: 448
variable_sites: 12
pi: 0.0149
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.97
markerseek_score: 68.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaI-ycf4_p1	ATTTCCGGCAATTGCAATGG	TCGAGACCCCGCTATAAGTT	45.0	50.0	58.0	57.9	546-561 bp	1.0	83.2
psaI-ycf4_p2	ATTTCCGGCAATTGCAATGG	TTCGAGACCCCGCTATAAGT	45.0	50.0	58.0	57.9	547-562 bp	1.0	83.2
psaI-ycf4_p3	GGGCTTAGTATTTCCGGCAA	TCGAGACCCCGCTATAAGTT	50.0	50.0	58.0	57.9	555-570 bp	1.0	82.9
psaI-ycf4_p4	GGGCTTAGTATTTCCGGCAA	TTCGAGACCCCGCTATAAGT	50.0	50.0	58.0	57.9	556-571 bp	1.0	82.9
psaI-ycf4_p5	GGCAATTGCAATGGCTTCTT	TCGAGACCCCGCTATAAGTT	45.0	50.0	57.9	57.9	540-555 bp	1.0	82.6