Hotspot

accD-psaI

LSC · 59027-59709 · 683 bp

Back to Flacourtia

Pi 0.00439

MarkerSeek score 62.70

Primer available yes

Label accD-psaI

Feature Metadata

label_name: accD-psaI
feature_type: igs
region: LSC
length_bp: 683
variable_sites: 5
pi: 0.00439
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 62.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
accD-psaI_p1	GCTCCACGCTTTCTTTCCTT	CCATTGCAATTGCCGGAAAT	50.0	45.0	58.8	58.0	808-841 bp	1.0	74.8
accD-psaI_p2	GCTCCACGCTTTCTTTCCTT	TTGCCGGAAATACTAAGCCC	50.0	50.0	58.8	58.0	799-832 bp	1.0	74.6
accD-psaI_p3	GCTCCACGCTTTCTTTCCTT	AAGAAGCCATTGCAATTGCC	50.0	45.0	58.8	57.9	814-847 bp	1.0	74.1
accD-psaI_p4	GCTCCACGCTTTCTTTCCT	CCATTGCAATTGCCGGAAAT	52.6	45.0	58.1	58.0	808-841 bp	1.0	72.3
accD-psaI_p5	TCCACGCTTTCTTTCCTTTGA	CCATTGCAATTGCCGGAAAT	42.9	45.0	58.1	58.0	806-839 bp	1.0	72.3