Hotspot

trnF-GAA-ndhJ

LSC · 47854-48604 · 751 bp

Back to Flacourtia

Pi 0.00361

MarkerSeek score 64.80

Primer available yes

Label trnF-GAA-ndhJ

Feature Metadata

label_name: trnF-GAA-ndhJ
feature_type: igs
region: LSC
length_bp: 751
variable_sites: 5
pi: 0.00361
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnF-GAA-ndhJ_p1	GGGATAGCTCAGCAGGTAGA	CCGGAAAGTTGGATAGGGTG	55.0	55.0	58.0	58.0	871-903 bp	1.0	82.7
trnF-GAA-ndhJ_p2	GGGATAGCTCAGCAGGTAGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	58.0	58.0	870-902 bp	1.0	82.7
trnF-GAA-ndhJ_p3	CCTCGTGTCACCAGTTCAAA	CCGGAAAGTTGGATAGGGTG	50.0	55.0	58.1	58.0	835-867 bp	1.0	82.5
trnF-GAA-ndhJ_p4	CCTCGTGTCACCAGTTCAAA	CGGAAAGTTGGATAGGGTGG	50.0	55.0	58.1	58.0	834-866 bp	1.0	82.5
trnF-GAA-ndhJ_p5	CAGGTAGAGCAGAGGACTGA	CGGAAAGTTGGATAGGGTGG	55.0	55.0	57.9	58.0	858-890 bp	1.0	81.7