Hotspot

lhbA-trnG-UCC

LSC · 35972-36425 · 454 bp

Back to Flacourtia

Pi 0.00965
MarkerSeek score 63.20
Primer available yes
Label lhbA-trnG-UCC

Feature Metadata

label_name
lhbA-trnG-UCC
feature_type
igs
region
LSC
length_bp
454
variable_sites
7
pi
0.00965
unique_haplotype_count
4
primer_available
yes
alignment_reliability
0.99
markerseek_score
63.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
lhbA-trnG-UCC_p1 CGGTTGGTCAAGCAACAAAA CGTCTTCTCCTTGGCAAAGA 45.0 50.0 57.7 57.8 598-631 bp 1.0 78.0
lhbA-trnG-UCC_p2 TTTCTTCTCCTGACGGTTGG CGTCTTCTCCTTGGCAAAGA 50.0 50.0 57.5 57.8 611-644 bp 1.0 75.9
lhbA-trnG-UCC_p3 TTCTTCTCCTGACGGTTGGT CGTCTTCTCCTTGGCAAAGA 50.0 50.0 58.6 57.8 610-643 bp 1.0 75.7
lhbA-trnG-UCC_p4 ACGGTTGGTCAAGCAACAAA CGTCTTCTCCTTGGCAAAGA 45.0 50.0 58.8 57.8 599-632 bp 1.0 73.8
lhbA-trnG-UCC_p5 TCTTCTCCTGACGGTTGGTC CGTCTTCTCCTTGGCAAAGA 55.0 50.0 59.0 57.8 609-642 bp 1.0 72.0