Hotspot

trnH-GUG-psbA

LSC · 76-342 · 267 bp

Back to Flacourtia

Pi 0.0599
MarkerSeek score 86.80
Primer available yes
Label trnH-GUG-psbA

Feature Metadata

label_name
trnH-GUG-psbA
feature_type
igs
region
LSC
length_bp
267
variable_sites
29
pi
0.0599
unique_haplotype_count
4
primer_available
yes
alignment_reliability
1.00
markerseek_score
86.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnH-GUG-psbA_p1 GATCCACTTGGCTACATCCG AACCGTGCAAACCTTGGTAT 55.0 45.0 58.1 58.0 380-403 bp 1.0 88.3
trnH-GUG-psbA_p2 CAATCCACTGCCTTGATCCA AACCGTGCAAACCTTGGTAT 50.0 45.0 57.9 58.0 394-417 bp 1.0 88.0
trnH-GUG-psbA_p3 AATCCACTGCCTTGATCCAC AACCGTGCAAACCTTGGTAT 50.0 45.0 57.9 58.0 393-416 bp 1.0 88.0
trnH-GUG-psbA_p4 ACAATCCACTGCCTTGATCC AACCGTGCAAACCTTGGTAT 50.0 45.0 57.9 58.0 395-418 bp 1.0 87.9
trnH-GUG-psbA_p5 GCGAATGACGGGAATTGAAC AACCGTGCAAACCTTGGTAT 50.0 45.0 57.8 58.0 432-455 bp 1.0 86.9