Hotspot

ndhF

SSC · 115853-118063 · 2211 bp

Back to Firmiana

Pi 0.00341

MarkerSeek score 39.70

Primer available yes

Label ndhF

Feature Metadata

label_name: ndhF
feature_type: gene
region: SSC
length_bp: 2211
variable_sites: 21
pi: 0.00341
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 39.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF_p1	CCCGGAACTTCTTCGTAGTG	AGAGCCGGTGAATCAGAAAC	55.0	50.0	58.0	57.9	2679-2744 bp	1.0	81.8
ndhF_p2	CCGGAACTTCTTCGTAGTGG	AGAGCCGGTGAATCAGAAAC	55.0	50.0	58.0	57.9	2678-2743 bp	1.0	81.8
ndhF_p3	GAGCTACTGAGGAACAACGG	AGAGCCGGTGAATCAGAAAC	55.0	50.0	58.0	57.9	2774-2839 bp	1.0	81.7
ndhF_p4	CCCCGATGTGTCATTGACAT	AGAGCCGGTGAATCAGAAAC	50.0	50.0	58.0	57.9	2572-2637 bp	1.0	81.6
ndhF_p5	GGGTTACCTGCTCTTAACGG	AGAGCCGGTGAATCAGAAAC	55.0	50.0	58.0	57.9	2911-2976 bp	1.0	81.5