Hotspot

atpA

LSC · 10303-11826 · 1524 bp

Back to Firmiana

Pi 0.00197

MarkerSeek score 31.20

Primer available yes

Label atpA

Feature Metadata

label_name: atpA
feature_type: gene
region: LSC
length_bp: 1524
variable_sites: 9
pi: 0.00197
unique_haplotype_count: 2
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 31.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
atpA_p1	AGCCTTCCAAGCTAACGATG	ATTGGCTTGTTTGGGGTGAT	50.0	45.0	58.0	58.0	2111-2224 bp	1.0	84.7
atpA_p2	CCTAGCCTTCCAAGCTAACG	ATTGGCTTGTTTGGGGTGAT	55.0	45.0	58.1	58.0	2114-2227 bp	1.0	84.4
atpA_p3	AGCCTTCCAAGCTAACGATG	AATGGAGGCGGATCAGTTTC	50.0	50.0	58.0	58.0	2361-2474 bp	1.0	84.1
atpA_p4	AGCCTTCCAAGCTAACGATG	GAAATGGAGGCGGATCAGTT	50.0	50.0	58.0	58.0	2363-2476 bp	1.0	84.1
atpA_p5	CCTAGCCTTCCAAGCTAACG	AATGGAGGCGGATCAGTTTC	55.0	50.0	58.1	58.0	2364-2477 bp	1.0	83.8