Hotspot

ccsA-ndhD

SSC · 121351-121633 · 283 bp

Back to Firmiana

Pi 0.0205

MarkerSeek score 52.20

Primer available yes

Label ccsA-ndhD

Feature Metadata

label_name: ccsA-ndhD
feature_type: igs
region: SSC
length_bp: 283
variable_sites: 16
pi: 0.0205
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.20

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ccsA-ndhD_p1	TTGGACCGTATTCGCGATTT	TGGACCGCGAGAGTTATTTG	45.0	50.0	58.0	58.0	589-618 bp	1.0	83.5
ccsA-ndhD_p2	TTGGACCGTATTCGCGATTT	GGACCGCGAGAGTTATTTGT	45.0	50.0	58.0	58.0	588-617 bp	1.0	83.5
ccsA-ndhD_p3	GTAAATTCCGCACTTGTGGC	TGGACCGCGAGAGTTATTTG	50.0	50.0	58.0	58.0	534-563 bp	1.0	83.5
ccsA-ndhD_p4	GTAAATTCCGCACTTGTGGC	GGACCGCGAGAGTTATTTGT	50.0	50.0	58.0	58.0	533-562 bp	1.0	83.5
ccsA-ndhD_p5	TTACTTGGACCGTATTCGCG	TGGACCGCGAGAGTTATTTG	50.0	50.0	58.1	58.0	593-622 bp	1.0	82.9