Hotspot

ndhF-rpl32

SSC · 118064-118923 · 860 bp

Back to Firmiana

Pi 0.0117

MarkerSeek score 52.90

Primer available yes

Label ndhF-rpl32

Feature Metadata

label_name: ndhF-rpl32
feature_type: igs
region: SSC
length_bp: 860
variable_sites: 27
pi: 0.0117
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 52.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ndhF-rpl32_p1	GGGAAGTGGCACAAAAGGTA	GGTTGAAAGCAAGGGAAGGA	50.0	50.0	58.0	58.0	1397-1446 bp	1.0	85.7
ndhF-rpl32_p2	TGTTGCCGTCGGAAAAAGTA	GGTTGAAAGCAAGGGAAGGA	45.0	50.0	58.0	58.0	1445-1494 bp	1.0	85.4
ndhF-rpl32_p3	GGGAAGTGGCACAAAAGGTA	TGGGCGATGGGGAAAATAAG	50.0	50.0	58.0	57.9	1115-1196 bp	1.0	85.4
ndhF-rpl32_p4	GGTAGCCAAGCTCGAAAGAA	GGTTGAAAGCAAGGGAAGGA	50.0	50.0	57.9	58.0	1608-1657 bp	1.0	85.0
ndhF-rpl32_p5	AAGGTAGCCAAGCTCGAAAG	GGTTGAAAGCAAGGGAAGGA	50.0	50.0	57.9	58.0	1610-1659 bp	1.0	84.9