Hotspot

trnR-ACG-trnN-GUU

IRb · 114601-115252 · 652 bp

Back to Firmiana

Pi 0.00286

MarkerSeek score 57.70

Primer available yes

Label trnR-ACG-trnN-GUU

Feature Metadata

label_name: trnR-ACG-trnN-GUU
feature_type: igs
region: IRb
length_bp: 652
variable_sites: 5
pi: 0.00286
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 57.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-ACG-trnN-GUU_p1	AACAGATCCAGTGGAGACGA	CCGTTGTTCCTCAGTAGCTC	50.0	55.0	58.1	58.0	818-829 bp	1.0	81.4
trnR-ACG-trnN-GUU_p2	AACAGATCCAGTGGAGACGA	TGGGTTGAGAACGGGAATTG	50.0	50.0	58.1	58.1	859-870 bp	1.0	80.6
trnR-ACG-trnN-GUU_p3	CAGAGGATTAGAGCACGTGG	CCGTTGTTCCTCAGTAGCTC	55.0	55.0	57.8	58.0	782-793 bp	1.0	80.4
trnR-ACG-trnN-GUU_p4	AACAGATCCAGTGGAGACGA	GCTGTTAACTGACTGGTCGT	50.0	50.0	58.1	57.8	782-793 bp	1.0	80.3
trnR-ACG-trnN-GUU_p5	AACAGATCCAGTGGAGACGA	TGGTCGTAGGTTCGAATCCT	50.0	50.0	58.1	58.2	769-780 bp	1.0	80.3