Hotspot

rps19-rpl2

IRb · 90148-90282 · 135 bp

Back to Firmiana

Pi 0.0333

MarkerSeek score 66.50

Primer available yes

Label rps19-rpl2

Feature Metadata

label_name: rps19-rpl2
feature_type: igs
region: IRb
length_bp: 135
variable_sites: 12
pi: 0.0333
unique_haplotype_count: 4
primer_available: yes
alignment_reliability: 0.87
markerseek_score: 66.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps19-rpl2_p1	TGGTAGATGCTCTGGACCAA	TTGGGGTTATCCTGCACTTG	50.0	50.0	58.0	57.8	312-380 bp	1.0	84.8
rps19-rpl2_p2	ATGGTAGATGCTCTGGACCA	TTGGGGTTATCCTGCACTTG	50.0	50.0	57.8	57.8	313-381 bp	1.0	83.7
rps19-rpl2_p3	TGGTAGATGCTCTGGACCAA	CATTCTTCGTCGCCGTAGTA	50.0	50.0	58.0	57.5	252-320 bp	1.0	83.4
rps19-rpl2_p4	TGGTAGATGCTCTGGACCAA	GGGTTATCCTGCACTTGGAA	50.0	50.0	58.0	57.5	309-377 bp	1.0	82.5
rps19-rpl2_p5	TGGTAGATGCTCTGGACCAA	CTTGGGGTTATCCTGCACTT	50.0	50.0	58.0	57.5	313-381 bp	1.0	82.4