Hotspot

petD-rpoA

LSC · 83437-83688 · 252 bp

Back to Firmiana

Pi 0.0193

MarkerSeek score 57.60

Primer available yes

Label petD-rpoA

Feature Metadata

label_name: petD-rpoA
feature_type: igs
region: LSC
length_bp: 252
variable_sites: 10
pi: 0.0193
unique_haplotype_count: 6
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 57.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petD-rpoA_p1	GGTTGGGTATTGGAGCAACA	CAGAATTGCCTCCCAGGATC	50.0	55.0	58.1	58.0	455-466 bp	1.0	83.5
petD-rpoA_p2	TGGTTGGGTATTGGAGCAAC	CAGAATTGCCTCCCAGGATC	50.0	55.0	58.1	58.0	456-467 bp	1.0	83.5
petD-rpoA_p3	GTTTCAGTACCTGCGGGATT	CAGAATTGCCTCCCAGGATC	50.0	55.0	57.9	58.0	576-587 bp	1.0	82.7
petD-rpoA_p4	GGTTGGGTATTGGAGCAACA	GCCTAACCGAACCGATTGAT	50.0	50.0	58.1	58.1	872-883 bp	1.0	81.2
petD-rpoA_p5	TGGTTGGGTATTGGAGCAAC	GCCTAACCGAACCGATTGAT	50.0	50.0	58.1	58.1	873-884 bp	1.0	81.2