Hotspot

psaJ-rpl33

LSC · 72395-72915 · 521 bp

Back to Firmiana

Pi 0.00614

MarkerSeek score 56.80

Primer available yes

Label psaJ-rpl33

Feature Metadata

label_name: psaJ-rpl33
feature_type: igs
region: LSC
length_bp: 521
variable_sites: 9
pi: 0.00614
unique_haplotype_count: 5
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 56.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
psaJ-rpl33_p1	TTCCCGGATGCGTTAACATT	ATACCTGGAAATGCCCGTTG	45.0	50.0	57.9	58.2	642-656 bp	1.0	79.6
psaJ-rpl33_p2	GTTTCTTCCCGGATGCGTTA	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	647-661 bp	1.0	78.4
psaJ-rpl33_p3	TTCTTCCCGGATGCGTTAAC	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	645-659 bp	1.0	78.4
psaJ-rpl33_p4	CGGATGCGTTAACATTCCCT	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	638-652 bp	1.0	77.9
psaJ-rpl33_p5	CTTCCCGGATGCGTTAACAT	ATACCTGGAAATGCCCGTTG	50.0	50.0	58.3	58.2	643-657 bp	1.0	77.9