Hotspot

ycf3-trnS-GGA

LSC · 47052-48048 · 997 bp

Back to Firmiana

Pi 0.00736
MarkerSeek score 52.50
Primer available yes
Label ycf3-trnS-GGA

Feature Metadata

label_name
ycf3-trnS-GGA
feature_type
igs
region
LSC
length_bp
997
variable_sites
22
pi
0.00736
unique_haplotype_count
5
primer_available
yes
alignment_reliability
1.00
markerseek_score
52.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ycf3-trnS-GGA_p1 TCTCCTGAGGTTGTCGGAAT TTCCAATGCTACGCCTTGAA 50.0 45.0 58.1 57.8 1118-1138 bp 1.0 80.5
ycf3-trnS-GGA_p2 TCTCCTGAGGTTGTCGGAAT GTGCGGAAAGAGAGGGATTC 50.0 55.0 58.1 58.3 1170-1190 bp 1.0 79.2
ycf3-trnS-GGA_p3 TTCTCCTGAGGTTGTCGGAA TTCCAATGCTACGCCTTGAA 50.0 45.0 58.3 57.8 1119-1139 bp 1.0 78.7
ycf3-trnS-GGA_p4 TTTCTCCTGAGGTTGTCGGA TTCCAATGCTACGCCTTGAA 50.0 45.0 58.3 57.8 1120-1140 bp 1.0 78.7
ycf3-trnS-GGA_p5 TCTCCTGAGGTTGTCGGAAT AGTTCCAATGCTACGCCTTG 50.0 50.0 58.1 58.5 1120-1140 bp 1.0 77.7