Hotspot

ycf1

SSC · 123933-129308 · 5376 bp

Back to Filipendula

Pi 0.0108

MarkerSeek score 47.90

Primer available yes

Label ycf1

Feature Metadata

label_name: ycf1
feature_type: gene
region: SSC
length_bp: 5376
variable_sites: 220
pi: 0.0108
unique_haplotype_count: 8
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 47.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
ycf1_p1	GTTGTCGGTGGCAGATAGAT	TCGGAAATTGATTTCGCCCA	50.0	45.0	57.4	58.2	725-731 bp	0.8	69.4
ycf1_p2	ACATACTGAAACGACTGCCA	TCGGAAATTGATTTCGCCCA	45.0	45.0	57.2	58.2	694-700 bp	0.8	67.5
ycf1_p3	TAATTCGTTGTCGGTGGCAG	TCGGAAATTGATTTCGCCCA	50.0	45.0	58.6	58.2	731-737 bp	0.7	65.6
ycf1_p4	GTAATTCGTTGTCGGTGGCA	TCGGAAATTGATTTCGCCCA	50.0	45.0	58.9	58.2	738 bp	0.6	57.4
ycf1_p5	TGTAATTCGTTGTCGGTGGC	TCGGAAATTGATTTCGCCCA	50.0	45.0	58.9	58.2	739 bp	0.6	57.4