Hotspot

trnV-GAC-rps12

IRa · 138453-141401 · 2949 bp

Back to Filipendula

Pi 0.0159
MarkerSeek score 64.50
Primer available yes
Label trnV-GAC-rps12

Feature Metadata

label_name
trnV-GAC-rps12
feature_type
igs
region
IRa
length_bp
2949
variable_sites
148
pi
0.0159
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnV-GAC-rps12_p1 ACCACTGAGTTATATCCCT AAGGCAGAGTTTGGTTTTT 42.1 36.8 51.6 53.1 2946-3000 bp 0.9 41.2
trnV-GAC-rps12_p2 TACCACTGAGTTATATCCCT AGGCAGAGTTTGGTTTTT 40.0 38.9 51.8 52.0 2946-3000 bp 0.9 41.2
trnV-GAC-rps12_p3 ACCACTGAGTTATATCCCT AAGGCAGAGTTTGGTTTT 42.1 38.9 51.6 52.0 2946-3000 bp 0.9 41.2
trnV-GAC-rps12_p4 ACCACTGAGTTATATCCCT AGGCAGAGTTTGGTTTTT 42.1 38.9 51.6 52.0 2945-2999 bp 0.9 41.2
trnV-GAC-rps12_p5 TACCACTGAGTTATATCCCT AGGCAGAGTTTGGTTTT 40.0 41.2 51.8 50.8 2946-3000 bp 0.9 41.2