Hotspot

trnL-UAG-ccsA

SSC · 114311-114425 · 115 bp

Back to Filipendula

Pi 0.023

MarkerSeek score 64.50

Primer available yes

Label trnL-UAG-ccsA

Feature Metadata

label_name: trnL-UAG-ccsA
feature_type: igs
region: SSC
length_bp: 115
variable_sites: 7
pi: 0.023
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnL-UAG-ccsA_p1	GTGAAATCGGTAGACACGCT	AGTCCTACGATTTCATCAACT	50.0	38.1	58.0	54.4	275-306 bp	1.0	50.6
trnL-UAG-ccsA_p2	TGAAATCGGTAGACACGCTG	AGTCCTACGATTTCATCAACT	50.0	38.1	58.0	54.4	274-305 bp	1.0	50.6
trnL-UAG-ccsA_p3	TAGACACGCTGCTCTTAGGA	AGTCCTACGATTTCATCAACT	50.0	38.1	57.9	54.4	265-296 bp	1.0	49.8
trnL-UAG-ccsA_p4	CGAATAAGCCGCTATGGTGA	AGTCCTACGATTTCATCAACT	50.0	38.1	57.9	54.4	291-322 bp	1.0	49.3
trnL-UAG-ccsA_p5	TCGAATAAGCCGCTATGGTG	AGTCCTACGATTTCATCAACT	50.0	38.1	57.9	54.4	292-323 bp	1.0	49.3