Hotspot

ndhF-trnL-UAG

SSC · 113021-114230 · 1210 bp

Back to Filipendula

Pi 0.0207
MarkerSeek score 64.80
Primer available yes
Label ndhF-trnL-UAG

Feature Metadata

label_name
ndhF-trnL-UAG
feature_type
igs
region
SSC
length_bp
1210
variable_sites
90
pi
0.0207
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-trnL-UAG_p1 CTGGAAGTGGAATAAAAGGT AGCGTGTCTACCGATTTCAC 40.0 50.0 52.7 58.0 1230-1307 bp 1.0 49.8
ndhF-trnL-UAG_p2 CTGGAAGTGGAATAAAAGGT CAGCGTGTCTACCGATTTCA 40.0 50.0 52.7 58.0 1231-1308 bp 1.0 49.8
ndhF-trnL-UAG_p3 CTGGAAGTGGAATAAAAGGT TCCTAAGAGCAGCGTGTCTA 40.0 50.0 52.7 57.9 1240-1317 bp 1.0 49.7
ndhF-trnL-UAG_p4 CTGGAAGTGGAATAAAAGGT CCTAAGAGCAGCGTGTCTAC 40.0 55.0 52.7 57.8 1239-1316 bp 1.0 49.7
ndhF-trnL-UAG_p5 CTGGAAGTGGAATAAAAGGT CTAAGAGCAGCGTGTCTACC 40.0 55.0 52.7 57.8 1238-1315 bp 1.0 49.7