Hotspot

rps12-trnV-GAC

IRb · 96503-99451 · 2949 bp

Back to Filipendula

Pi 0.0159
MarkerSeek score 64.50
Primer available yes
Label rps12-trnV-GAC

Feature Metadata

label_name
rps12-trnV-GAC
feature_type
igs
region
IRb
length_bp
2949
variable_sites
148
pi
0.0159
unique_haplotype_count
8
primer_available
yes
alignment_reliability
1.00
markerseek_score
64.50

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps12-trnV-GAC_p1 AAGGCAGAGTTTGGTTTTT ACCACTGAGTTATATCCCT 36.8 42.1 53.1 51.6 2946-3000 bp 0.9 41.2
rps12-trnV-GAC_p2 AGGCAGAGTTTGGTTTTT TACCACTGAGTTATATCCCT 38.9 40.0 52.0 51.8 2946-3000 bp 0.9 41.2
rps12-trnV-GAC_p3 AAGGCAGAGTTTGGTTTT ACCACTGAGTTATATCCCT 38.9 42.1 52.0 51.6 2946-3000 bp 0.9 41.2
rps12-trnV-GAC_p4 AGGCAGAGTTTGGTTTTT ACCACTGAGTTATATCCCT 38.9 42.1 52.0 51.6 2945-2999 bp 0.9 41.2
rps12-trnV-GAC_p5 AGGCAGAGTTTGGTTTT TACCACTGAGTTATATCCCT 41.2 40.0 50.8 51.8 2946-3000 bp 0.9 41.2