Hotspot

rps3-rpl22

LSC · 81889-82303 · 415 bp

Back to Filipendula

Pi 0.0189

MarkerSeek score 51.90

Primer available yes

Label rps3-rpl22

Feature Metadata

label_name: rps3-rpl22
feature_type: igs
region: LSC
length_bp: 415
variable_sites: 34
pi: 0.0189
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 51.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rps3-rpl22_p1	TTGTACTTGCCTGTTCGGTT	AAAACCCCGAGCTAGTAGGT	45.0	50.0	57.7	58.1	747 bp	0.5	58.0
rps3-rpl22_p2	TTTGTACTTGCCTGTTCGGT	AAAACCCCGAGCTAGTAGGT	45.0	50.0	57.7	58.1	748 bp	0.5	58.0
rps3-rpl22_p3	TGTGAAGCGGTAGGTTGTTT	AAAACCCCGAGCTAGTAGGT	45.0	50.0	57.7	58.1	1154-1155 bp	0.5	57.9
rps3-rpl22_p4	TTGTACTTGCCTGTTCGGTT	TCTAAATTTCCGGGGGCATG	45.0	50.0	57.7	57.9	1202 bp	0.5	57.7
rps3-rpl22_p5	TTTGTACTTGCCTGTTCGGT	TCTAAATTTCCGGGGGCATG	45.0	50.0	57.7	57.9	1203 bp	0.5	57.7