Hotspot

trnS-GGA-ycf3

LSC · 61312-62179 · 868 bp

Back to Filipendula

Pi 0.015

MarkerSeek score 65.60

Primer available yes

Label trnS-GGA-ycf3

Feature Metadata

label_name: trnS-GGA-ycf3
feature_type: igs
region: LSC
length_bp: 868
variable_sites: 50
pi: 0.015
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 1.00
markerseek_score: 65.60

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnS-GGA-ycf3_p1	ACCCTCGGTAAACAAACGTC	TTCTCCTGAAGTTGTCGGAA	50.0	45.0	58.1	56.4	1014-1050 bp	0.9	67.9
trnS-GGA-ycf3_p2	ACCCTCGGTAAACAAACGTC	TTTCTCCTGAAGTTGTCGGA	50.0	45.0	58.1	56.4	1015-1051 bp	0.9	67.9
trnS-GGA-ycf3_p3	ACGGAAAGAGAGGGATTCGA	TTCTCCTGAAGTTGTCGGAA	50.0	45.0	58.2	56.4	1034-1070 bp	0.9	67.7
trnS-GGA-ycf3_p4	CCCTCGGTAAACAAACGTCT	TTCTCCTGAAGTTGTCGGAA	50.0	45.0	57.8	56.4	1013-1049 bp	0.9	67.7
trnS-GGA-ycf3_p5	ACGGAAAGAGAGGGATTCGA	TTTCTCCTGAAGTTGTCGGA	50.0	45.0	58.2	56.4	1035-1071 bp	0.9	67.6