Hotspot

rbcL-psbM

LSC · 52969-53788 · 820 bp

Back to Filipendula

Pi 0.0134

MarkerSeek score 68.70

Primer available yes

Label rbcL-psbM

Feature Metadata

label_name: rbcL-psbM
feature_type: igs
region: LSC
length_bp: 820
variable_sites: 42
pi: 0.0134
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 68.70

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
rbcL-psbM_p1	CTTGCTCGTGAGGGTAATGA	GTAAAAACAGTCAGCCAAGGC	50.0	47.6	57.3	58.0	952-1029 bp	1.0	73.0
rbcL-psbM_p2	TGCTGCTTGTGAAGTATGGA	GTAAAAACAGTCAGCCAAGGC	45.0	47.6	57.1	58.0	893-970 bp	1.0	71.9
rbcL-psbM_p3	CTTGCTCGTGAGGGTAATGA	CGTAAAAACAGTCAGCCAAGG	50.0	47.6	57.3	57.7	953-1030 bp	1.0	70.9
rbcL-psbM_p4	AGTCCTGAACTATCTGCTGC	GTAAAAACAGTCAGCCAAGGC	50.0	47.6	57.0	58.0	907-984 bp	1.0	70.8
rbcL-psbM_p5	GTCCTGAACTATCTGCTGCT	GTAAAAACAGTCAGCCAAGGC	50.0	47.6	57.0	58.0	906-983 bp	1.0	70.8