Hotspot

petN-ndhC

LSC · 28637-30030 · 1394 bp

Back to Filipendula

Pi 0.0172

MarkerSeek score 63.00

Primer available yes

Label petN-ndhC

Feature Metadata

label_name: petN-ndhC
feature_type: igs
region: LSC
length_bp: 1394
variable_sites: 91
pi: 0.0172
unique_haplotype_count: 9
primer_available: yes
alignment_reliability: 0.99
markerseek_score: 63.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
petN-ndhC_p1	TCTTGCTTGGGCTGCTTTAA	TCATATTCGTAAAGCAGAAGCA	45.0	36.4	58.0	55.9	1296-1493 bp	1.0	56.3
petN-ndhC_p2	ATCTTGCTTGGGCTGCTTTA	TCATATTCGTAAAGCAGAAGCA	45.0	36.4	57.8	55.9	1297-1494 bp	1.0	54.6
petN-ndhC_p3	TAAATCTTGCTTGGGCTGCT	TCATATTCGTAAAGCAGAAGCA	45.0	36.4	57.8	55.9	1300-1497 bp	1.0	54.6
petN-ndhC_p4	GTAAATCTTGCTTGGGCTGC	TCATATTCGTAAAGCAGAAGCA	50.0	36.4	57.7	55.9	1301-1498 bp	1.0	54.1
petN-ndhC_p5	ATGGGGAAGAAGTGGACTCT	TCATATTCGTAAAGCAGAAGCA	50.0	36.4	57.6	55.9	1242-1439 bp	1.0	53.8