Hotspot

trnR-UCU-atpA

LSC · 9975-10493 · 519 bp

Back to Filipendula

Pi 0.0179

MarkerSeek score 69.90

Primer available yes

Label trnR-UCU-atpA

Feature Metadata

label_name: trnR-UCU-atpA
feature_type: igs
region: LSC
length_bp: 519
variable_sites: 32
pi: 0.0179
unique_haplotype_count: 7
primer_available: yes
alignment_reliability: 0.96
markerseek_score: 69.90

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID	fwd_seq	rev_seq	fwd_gc	rev_gc	fwd_tm	rev_tm	amplicon_size	cross_species_success_rate	primer_score
trnR-UCU-atpA_p1	GGTTCAAATCCTATTGGACGC	CCAAGGAAGCAGAAACCCTT	47.6	50.0	57.3	58.0	595-629 bp	0.9	70.7
trnR-UCU-atpA_p2	TGGATAGGACAGAGGTCTTCT	CCAAGGAAGCAGAAACCCTT	47.6	50.0	56.9	58.0	630-664 bp	0.9	67.8
trnR-UCU-atpA_p3	GGTTCAAATCCTATTGGACGC	TACCAAGGAAGCAGAAACCC	47.6	50.0	57.3	57.4	597-631 bp	0.9	66.1
trnR-UCU-atpA_p4	GTTCAAATCCTATTGGACGCA	CCAAGGAAGCAGAAACCCTT	42.9	50.0	56.6	58.0	594-628 bp	0.9	65.1
trnR-UCU-atpA_p5	ATGGATAGGACAGAGGTCTTCT	CCAAGGAAGCAGAAACCCTT	45.5	50.0	57.4	58.0	631-665 bp	0.9	63.8