Hotspot

rps16-trnQ-UUG

LSC · 6300-7078 · 779 bp

Back to Filipendula

Pi 0.0212
MarkerSeek score 64.80
Primer available yes
Label rps16-trnQ-UUG

Feature Metadata

label_name
rps16-trnQ-UUG
feature_type
igs
region
LSC
length_bp
779
variable_sites
55
pi
0.0212
unique_haplotype_count
7
primer_available
yes
alignment_reliability
0.99
markerseek_score
64.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rps16-trnQ-UUG_p1 CCACAACGGATCATGTCCTT GAGGTTCGAATCCTTCCGTC 50.0 55.0 57.9 58.1 859-873 bp 1.0 84.9
rps16-trnQ-UUG_p2 AAGTCGCACGTTGCTTTCTA GAGGTTCGAATCCTTCCGTC 45.0 55.0 58.1 58.1 838-852 bp 1.0 84.9
rps16-trnQ-UUG_p3 TCATGTCCTTCAAGTCGCAC GAGGTTCGAATCCTTCCGTC 50.0 55.0 58.2 58.1 849-863 bp 1.0 84.3
rps16-trnQ-UUG_p4 CCACAACGGATCATGTCCTT TTCGGAGGTTCGAATCCTTC 50.0 50.0 57.9 57.3 863-877 bp 1.0 80.2
rps16-trnQ-UUG_p5 CCACAACGGATCATGTCCTT TAAGGCAACGGGTTTTGGTC 50.0 50.0 57.9 58.7 889-903 bp 1.0 79.9