Hotspot

rpl32-trnL-UAG

SSC · 118560-120063 · 1504 bp

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Pi 0.0158
MarkerSeek score 75.00
Primer available yes
Label rpl32-trnL-UAG

Feature Metadata

label_name
rpl32-trnL-UAG
feature_type
igs
region
SSC
length_bp
1504
variable_sites
162
pi
0.0158
unique_haplotype_count
38
primer_available
yes
alignment_reliability
0.98
markerseek_score
75.00

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
rpl32-trnL-UAG_p1 GGGCAGCATTGAAAGCTTTT AGCGTGTCTACCGATTTCAC 45.0 50.0 57.8 58.0 1616-1902 bp 0.97561 88.5
rpl32-trnL-UAG_p2 GGGCAGCATTGAAAGCTTTT CAGCGTGTCTACCGATTTCA 45.0 50.0 57.8 58.0 1617-1903 bp 0.97561 88.5
rpl32-trnL-UAG_p3 GGGCAGCATTGAAAGCTTTT TCCTAAGAGCAGCGTGTCTA 45.0 50.0 57.8 57.9 1626-1912 bp 0.97561 87.5
rpl32-trnL-UAG_p4 GGGCAGCATTGAAAGCTTTT CCTAAGAGCAGCGTGTCTAC 45.0 55.0 57.8 57.8 1625-1911 bp 0.97561 86.9
rpl32-trnL-UAG_p5 GGGCAGCATTGAAAGCTTTT CTAAGAGCAGCGTGTCTACC 45.0 55.0 57.8 57.8 1624-1910 bp 0.97561 86.9