Hotspot

ndhF-rpl32

SSC · 117016-118400 · 1385 bp

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Pi 0.017
MarkerSeek score 81.80
Primer available yes
Label ndhF-rpl32

Feature Metadata

label_name
ndhF-rpl32
feature_type
igs
region
SSC
length_bp
1385
variable_sites
138
pi
0.017
unique_haplotype_count
39
primer_available
yes
alignment_reliability
0.99
markerseek_score
81.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
ndhF-rpl32_p1 GGCGAAGATCTTTTGTTGCC AAAAGCTTTCAATGCTGCCC 50.0 45.0 58.0 57.8 1564-1642 bp 1.0 89.7
ndhF-rpl32_p2 CCACATACGGCGAAGATCTT AAAAGCTTTCAATGCTGCCC 50.0 45.0 57.8 57.8 1572-1650 bp 1.0 88.0
ndhF-rpl32_p3 CGGCGAAGATCTTTTGTTGC AAAAGCTTTCAATGCTGCCC 50.0 45.0 58.4 57.8 1565-1643 bp 1.0 86.7
ndhF-rpl32_p4 GCGAAGATCTTTTGTTGCCG AAAAGCTTTCAATGCTGCCC 50.0 45.0 58.4 57.8 1563-1641 bp 1.0 86.7
ndhF-rpl32_p5 GGCGAAGATCTTTTGTTGCC AGCTTTCAATGCTGCCCAAT 50.0 45.0 58.0 58.7 1561-1639 bp 1.0 85.3