Hotspot

psbZ-trnG-GCC

LSC · 38683-39355 · 673 bp

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Pi 0.0125
MarkerSeek score 70.10
Primer available yes
Label psbZ-trnG-GCC

Feature Metadata

label_name
psbZ-trnG-GCC
feature_type
igs
region
LSC
length_bp
673
variable_sites
66
pi
0.0125
unique_haplotype_count
37
primer_available
yes
alignment_reliability
0.98
markerseek_score
70.10

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
psbZ-trnG-GCC_p1 TTGCTTCTCCTGATGGTTGG AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 593-868 bp 1.0 88.6
psbZ-trnG-GCC_p2 TTGCTTCTCCTGATGGTTGG GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 594-869 bp 1.0 88.6
psbZ-trnG-GCC_p3 GCTTCTCCTGATGGTTGGTT AATCGAACCCGCATCTTCTC 50.0 50.0 57.8 58.1 591-866 bp 1.0 88.6
psbZ-trnG-GCC_p4 GCTTCTCCTGATGGTTGGTT GAATCGAACCCGCATCTTCT 50.0 50.0 57.8 58.1 592-867 bp 1.0 88.6
psbZ-trnG-GCC_p5 TCCTGATGGTTGGTTGGGTA AATCGAACCCGCATCTTCTC 50.0 50.0 58.3 58.1 586-861 bp 1.0 88.3