Hotspot

trnC-GCA-petN

LSC · 29660-30687 · 1028 bp

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Pi 0.0112
MarkerSeek score 72.80
Primer available yes
Label trnC-GCA-petN

Feature Metadata

label_name
trnC-GCA-petN
feature_type
igs
region
LSC
length_bp
1028
variable_sites
83
pi
0.0112
unique_haplotype_count
33
primer_available
yes
alignment_reliability
0.99
markerseek_score
72.80

Pi Curve

Haplotype Network

Species Separation

Primer Ranking

Primer ID fwd_seq rev_seq fwd_gc rev_gc fwd_tm rev_tm amplicon_size cross_species_success_rate primer_score
trnC-GCA-petN_p1 TTTCCCCAGTTCAAATCCGG AGCCCAAGCGAGACTTACTA 50.0 50.0 58.1 58.2 1036-1084 bp 1.0 88.3
trnC-GCA-petN_p2 TTAGATCGTTTTGGCGGCAT AGCCCAAGCGAGACTTACTA 45.0 50.0 58.3 58.2 1090-1138 bp 1.0 86.6
trnC-GCA-petN_p3 TTTCCCCAGTTCAAATCCGG AGAGTCCACTTCTTCCCCAT 50.0 50.0 58.1 57.6 1097-1145 bp 1.0 86.4
trnC-GCA-petN_p4 GGGGGACTGCAAATCCTTTT AGCCCAAGCGAGACTTACTA 50.0 50.0 58.4 58.2 1054-1102 bp 1.0 86.0
trnC-GCA-petN_p5 TTAGATCGTTTTGGCGGCAT AGAGTCCACTTCTTCCCCAT 45.0 50.0 58.3 57.6 1151-1199 bp 1.0 84.7